diff --git a/autoload/ale/fixers/autopep8.vim b/autoload/ale/fixers/autopep8.vim
index 59130af8..92271336 100644
--- a/autoload/ale/fixers/autopep8.vim
+++ b/autoload/ale/fixers/autopep8.vim
@@ -2,7 +2,12 @@
 " Description: Fixing files with autopep8.
 
 function! ale#fixers#autopep8#Fix(buffer) abort
+    let l:executable = ale#handlers#python#GetExecutable(a:buffer, 'autopep8')
+    if empty(l:executable)
+        return 0
+    endif
+
     return {
-    \   'command': 'autopep8 -'
+    \   'command': ale#Escape(l:executable) . ' -'
     \}
 endfunction
diff --git a/autoload/ale/fixers/isort.vim b/autoload/ale/fixers/isort.vim
index 2d47434a..04830b28 100644
--- a/autoload/ale/fixers/isort.vim
+++ b/autoload/ale/fixers/isort.vim
@@ -2,12 +2,17 @@
 " Description: Fixing Python imports with isort.
 
 function! ale#fixers#isort#Fix(buffer) abort
+    let l:executable = ale#handlers#python#GetExecutable(a:buffer, 'isort')
+    if empty(l:executable)
+        return 0
+    endif
+
     let l:config = ale#path#FindNearestFile(a:buffer, '.isort.cfg')
     let l:config_options = !empty(l:config)
     \   ? ' --settings-path ' . ale#Escape(l:config)
     \   : ''
 
     return {
-    \   'command': 'isort' . l:config_options . ' -',
+    \   'command': ale#Escape(l:executable) . l:config_options . ' -',
     \}
 endfunction
diff --git a/autoload/ale/fixers/yapf.vim b/autoload/ale/fixers/yapf.vim
index 479fd75e..46da4081 100644
--- a/autoload/ale/fixers/yapf.vim
+++ b/autoload/ale/fixers/yapf.vim
@@ -2,12 +2,17 @@
 " Description: Fixing Python files with yapf.
 
 function! ale#fixers#yapf#Fix(buffer) abort
+    let l:executable = ale#handlers#python#GetExecutable(a:buffer, 'yapf')
+    if empty(l:executable)
+        return 0
+    endif
+
     let l:config = ale#path#FindNearestFile(a:buffer, '.style.yapf')
     let l:config_options = !empty(l:config)
     \   ? ' --style ' . ale#Escape(l:config)
     \   : ''
 
     return {
-    \   'command': 'yapf --no-local-style' . l:config_options,
+    \   'command': ale#Escape(l:executable) . ' --no-local-style' . l:config_options,
     \}
 endfunction
diff --git a/autoload/ale/handlers/python.vim b/autoload/ale/handlers/python.vim
index ae6f6d6c..58e7d757 100644
--- a/autoload/ale/handlers/python.vim
+++ b/autoload/ale/handlers/python.vim
@@ -45,3 +45,24 @@ function! ale#handlers#python#HandlePEP8Format(buffer, lines) abort
 
     return l:output
 endfunction
+
+" Given a buffer number and a command name, find the path to the executable.
+" First search on a virtualenv for Python, if nothing is found, try the global
+" command. Returns an empty string if cannot find the executable
+function! ale#handlers#python#GetExecutable(buffer, cmd_name) abort
+    let l:virtualenv = ale#python#FindVirtualenv(a:buffer)
+
+    if !empty(l:virtualenv)
+        let l:ve_executable = l:virtualenv . '/bin/' . a:cmd_name
+
+        if executable(l:ve_executable)
+            return l:ve_executable
+        endif
+    endif
+
+    if executable(a:cmd_name)
+        return a:cmd_name
+    endif
+
+    return ''
+endfunction
diff --git a/test/command_callback/python_paths/with_virtualenv/env/bin/autopep8 b/test/command_callback/python_paths/with_virtualenv/env/bin/autopep8
new file mode 100755
index 00000000..e69de29b
diff --git a/test/command_callback/python_paths/with_virtualenv/env/bin/isort b/test/command_callback/python_paths/with_virtualenv/env/bin/isort
new file mode 100755
index 00000000..e69de29b
diff --git a/test/command_callback/python_paths/with_virtualenv/env/bin/yapf b/test/command_callback/python_paths/with_virtualenv/env/bin/yapf
new file mode 100755
index 00000000..e69de29b
diff --git a/test/fixers/test_python_fixer_command_callback.vader b/test/fixers/test_python_fixer_command_callback.vader
new file mode 100644
index 00000000..59135d38
--- /dev/null
+++ b/test/fixers/test_python_fixer_command_callback.vader
@@ -0,0 +1,58 @@
+Before:
+  silent! execute 'cd /testplugin/test/command_callback'
+  let g:dir = getcwd()
+
+After:
+  " Set the file to something else,
+  " or we'll cause issues when running other tests
+  silent file 'dummy.py'
+  unlet! g:dir
+
+Execute(The python GetExecutable callbacks should return the correct path):
+  AssertEqual
+  \ '',
+  \ ale#handlers#python#GetExecutable(bufnr(''), 'isort')
+
+  silent execute 'file ' . fnameescape(g:dir . '/python_paths/with_virtualenv/subdir/foo/bar.py')
+  AssertEqual
+  \ g:dir . '/python_paths/with_virtualenv/env/bin/isort',
+  \ ale#handlers#python#GetExecutable(bufnr(''), 'isort')
+  AssertEqual
+  \ g:dir . '/python_paths/with_virtualenv/env/bin/autopep8',
+  \ ale#handlers#python#GetExecutable(bufnr(''), 'autopep8')
+  AssertEqual
+  \ g:dir . '/python_paths/with_virtualenv/env/bin/yapf',
+  \ ale#handlers#python#GetExecutable(bufnr(''), 'yapf')
+
+
+Execute(The autopep8 callbacks should return the correct default values):
+  AssertEqual
+  \ 0,
+  \ ale#fixers#autopep8#Fix(bufnr(''))
+
+  silent execute 'file ' . fnameescape(g:dir . '/python_paths/with_virtualenv/subdir/foo/bar.py')
+  AssertEqual
+  \ {'command': "'" . g:dir . "/python_paths/with_virtualenv/env/bin/autopep8' -" },
+  \ ale#fixers#autopep8#Fix(bufnr(''))
+
+
+Execute(The isort callbacks should return the correct default values):
+  AssertEqual
+  \ 0,
+  \ ale#fixers#isort#Fix(bufnr(''))
+
+  silent execute 'file ' . fnameescape(g:dir . '/python_paths/with_virtualenv/subdir/foo/bar.py')
+  AssertEqual
+  \ {'command': "'" . g:dir . "/python_paths/with_virtualenv/env/bin/isort' -" },
+  \ ale#fixers#isort#Fix(bufnr(''))
+
+
+Execute(The yapf callbacks should return the correct default values):
+  AssertEqual
+  \ 0,
+  \ ale#fixers#yapf#Fix(bufnr(''))
+
+  silent execute 'file ' . fnameescape(g:dir . '/python_paths/with_virtualenv/subdir/foo/bar.py')
+  AssertEqual
+  \ {'command': "'" . g:dir . "/python_paths/with_virtualenv/env/bin/yapf' --no-local-style" },
+  \ ale#fixers#yapf#Fix(bufnr(''))